http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103820362-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-50 |
| filingDate | 2014-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103820362-B |
| titleOfInvention | A kind of structure of biosynthesizing gentamicinX 2 engineering bacteria and application thereof |
| abstract | The invention belongs to medical art, relate to structure and the application thereof of biosynthesizing gentamicinX 2 genetic engineering bacterium.Utilize microbial molecules genetics technology, make the biosynthesizing key gene inactivation of gentamicin in deep red micromonospora, block gentamicin biosynthetic metabolism stream, directed accumulation gentamicinX 2.The present invention is by construction recombination plasmid pFU503, and pFU503 is imported deep red micromonospora GK1101, technology is knocked out by frame, the function of deactivation <i>genB3</iGreat T.GreaT.GT gene, screening double exchange bacterial strain, tunning detects, and new component qualification, confirms as gentamicinX 2 producing strains.Engineering bacteria constructed by the present invention, stabilization characteristics of genetics, can accumulate gentamicinX 2 in a large number, has filled up domestic and international research blank.Constructed engineering bacteria production technique is simple, and fermentation control is easy, and combined coefficient is high, and Hydrolysis kinetics is convenient, constant product quality, almost reaches clean production boundary, has important commercial application and is worth, can produce great economic benefit. |
| priorityDate | 2014-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.