http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103808825-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7ff3dd1559c1dcaa53105b631eaa37ed |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-64 |
| filingDate | 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_927dcd77861defc3934dfe0b40302470 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f277058dd6fbb7e8b7d8c278af65b75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98b45254e89dcc98221eee207703a966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1cf86611b939c1f799fae685e0a8751 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_083172f8abebb7cf32698381f3367bda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14340fdfc27764a3a9dfb20b00d354b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0dca01e7414396667f44c90679badd22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26b68374ecb127e2575817569c66a4ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a5e4336e1a38a7b9787a02199ac91ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_671cce0897f28d1860171755fd0cbf1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5df5fea94a335f2841bc5a8f7e9ddae6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8966eb38b2fa60b95bee1cb0f43d141e |
| publicationDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103808825-B |
| titleOfInvention | Method for detecting urotropin in cigarette mainstream smoke through high performance liquid chromatography-tandem mass spectrum |
| abstract | The invention claims a method for detecting urotropin in cigarette mainstream smoke through high performance liquid chromatography-tandem mass spectrum. The method comprises the steps of gathering mainstream smoke total particulate matter of 20 cigarettes according to the rule of ISO4387:2000, then putting a cambridge filter with the mainstream smoke total particulate matter into a 250mL bread bottle, adding 40 mL of ethanol extract liquor containing triethylamine to oscillate for extracting the cambridge filter for 40 minutes, then filtering through a 0.45 micron filter membrane to detect by using GC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry). Instrument conditions are as follows: a HILIC (Hydrophilic Interaction Chromatography) is used; the detection temperature is 30 DEG C; the sample size is 2microliter; the flow velocity is 0.25mL per minute; a ratio of a mobile phase V (acetonitrile) to V (5mmol/L ammonium acetate solution) is 75:25; the mobile phase carries out isocratic elution. Mass spectrum conditions are as follows: an ionization mode is electros spray; a scanning mode is positive ion; the atomizing gas is 40 psi; the curtain gas is 10 psi; the auxiliary trim gas is 40 psi; the collision gas is 5 psi; the ion source voltage is 4000-5000V; the ion source temperature is 250-350 DEG C. The method is simple to operate, good in separation effect, accurate to determine the nature, short in sample analysis time and high in detection sensitivity. |
| priorityDate | 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.