http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103808716-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate | 2014-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103808716-B |
titleOfInvention | A kind of method of portable quick detection ochratoxin A |
abstract | A kind of method that the invention discloses portable quick detection ochratoxin A. First invertase-Terminal Acetylenes modifying DNA is prepared. Subsequently biotin-nitrine modifying DNA is combined in magnetic bead surfaces, and adds the ochratoxin A mixing of variable concentrations. It is subsequently adding the invertase-Terminal Acetylenes modifying DNA, the CuSO that had previously prepared 4 Solution, ascorbic acid solution. Wherein ascorbic acid solution can reduce Cu (II) and obtains Cu (I) compound, and catalysis biological element-nitrine modifying DNA reacts with invertase-Terminal Acetylenes modifying DNA generation click chemistry. Reaction takes the supernatant and adds to sucrose solution after terminating, invertase can effectively in catalytic solution sucrose inversion be glucose. Blood glucose meter is finally adopted to be measured. The method organically will click on chemistry rapid-action advantage and combines with the feature of aptamers high specific, adopts simply portable blood glucose meter to carry out data acquisition, greatly reduces complexity and the testing cost of operation, improve sensitivity and selectivity. |
priorityDate | 2014-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.