http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103808701-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103808701-B |
titleOfInvention | Based on the ochratoxin A homogeneous phase method for quick of nucleic acid interca-lating dyes fluorescent quenching |
abstract | The invention discloses a kind of ochratoxin A homogeneous phase method for quick based on the fluorescent quenching of nucleic acid interca-lating dyes, comprise the following steps: the formation of (1) ochratoxin A aptamer and nucleic acid interca-lating dyes fluorescent composition; (2) ochratoxin A aptamer is to the specific recognition of ochratoxin A.The present invention adopts ochratoxin A aptamer as molecular recognition elements, the OTA detection method that nucleic acid interca-lating dyes builds as reporter molecules, greatly reduce the cost detecting OTA mycotoxin, favorable reproducibility, specificity is high, simple to operation and can realize the Parallel testing of a large amount of sample; The present invention is based on the ochratoxin A homogeneous phase method for quick of nucleic acid interca-lating dyes fluorescent quenching, after ochratoxin A aptamer and nucleic acid interca-lating dyes fluorescent composition are pre-formed, only need the detection that 5-10min can realize OTA. |
priorityDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.