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filingDate 2013-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4176cede47052fbc0171666415eaadc
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publicationDate 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103806109-A
titleOfInvention Excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and construction method thereof
abstract The invention relates to an excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and a construction method thereof. A high-quality excellent skiing sportsman enriched cDNA library with high titer, high recombination rate and large insertion fragment range is obtained by performing total RNA (Ribonucleic Acid) extraction, double-chain cDNA synthesis, digestion using protease K, Sfi1 enzyme digestion, connection into a vector and package of lambda phage. The invention belongs to the field of interdiscipline of sports science, human resource methodology, cell biology and molecular biology.
priorityDate 2013-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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