http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103805621-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2014-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103805621-B |
| titleOfInvention | The novel preparation process of targeting antineoplastic amalgamation protein matter LPO |
| abstract | nullThe invention discloses the novel preparation process of targeting antineoplastic amalgamation protein matter LPO，Build with transsulfurase A(TrxA) the auxiliary fragment expressed for protein solubility of-histidine-tagged (His6-Tag)-SUMO protease identification substrate，By LHRH-PEA transferring film peptide-ONC(referred to as LPO) for the purpose of the amalgamation and expression protein of fragment，And make this two parts protein be connected with carrier positive sequence with correct reading frame and convert entrance to express strain，The fusion protein of six kinds of material series connection of final construction and expression，After slightly carrying，Metal-chelating medium purification is carried out under imidazoles exists，SUMO protease enzyme action，The LPO albumen that the LPO protein of preparation is prepared than conventional method is to colon cancer cell line HT-29、Ovarian cancer OVCAR3 cell、The inhibitory action of the tumor cell lines such as adenocarcinoma of cervix HeLa cell and HepG-2 cell line is all significantly improved. |
| priorityDate | 2013-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 275.