http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103805564-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0781 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2012-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103805564-B |
| titleOfInvention | Prepare the chromosomal method of the precocious aggegation of human peripheral lymphocyte fast |
| abstract | The present invention relates to and prepare the chromosomal method of the precocious aggegation of human peripheral lymphocyte fast, its key step comprises: (1), blood sample are placed in 37 DEG C of constant incubators through phytohemagglutinin process and leave standstill 45-90 minute; (2) mixed-culture medium 37 DEG C, be placed in by the blood sample medium size lymphocyte after phytohaemagglutinin process containing colchicine, ATP, 5% foetal calf serum, CalyculinA and CDK1/CyclinB cultivates 3-12 hour; (3), after cell co-cultivation, 0.075mol is used? the hypotonic 10-20 minute of KCl; (4), add stationary liquid pre-fix 1 time in the hypotonic medium containing Hypotonic treatment cell, then add stationary liquid and fix 2 times, each fixing 10-15 minute, is finally suspended from cell in stationary liquid.The present invention, compared with the precocious aggegation method of chromosome preparation of routine, substantially reduces the time, only needs 3-12 hour. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106501040-A |
| priorityDate | 2012-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.