| abstract |
The invention discloses an aza-hybridized guanosine and its synthesis method and application in DNA sequencing; the synthesis method comprises the following steps: the compound of formula (Ⅲ) is deprotected under alkaline conditions to obtain the compound of formula (II) Compound; Further demethylation to obtain a compound of formula (I), i.e. 7-deaza-7-halogen-8-aza-guanosine; Wherein, R 1 is H or OH, R 2 is I, Br or Cl, R 3 is H or The aza-hybridized guanosine of the present invention is a novel reagent for DNA sequencing, and has better base recognition effect and more stable DNA chain structure than the 8-position unsubstituted nitrogen guanosine. At the same time, the synthesis method of 8-nitrogen substituted guanosine in the prior art is complicated, the yield is low, and has not yet been commercialized. However, the raw materials required by the synthesis method of the present invention are simple and easy to obtain, and the conventional chemical synthesis reaction is adopted, and the yield Higher, suitable for widespread promotion and use. |