http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103804293-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a843c3bc6ef616d46ada26b7194ec53 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D221-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D221-22 |
| filingDate | 2012-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b904206ae739917feb826ac519bb2ee3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63ec50194e2485950043249f7936b900 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03371dc3094262dd20e6e8c9b98aa935 |
| publicationDate | 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103804293-A |
| titleOfInvention | Ordinary pressure column chromatography combination method for separation and purification of huperzine A |
| abstract | A method for separation and purification of huperzine A by adopting an alkaline alumina and silica gel column chromatography combination method under the ordinary pressure is applicable to a crude extract of a herbal medicine huperzia serrata, and the content of huperzine A is not less than 1%. The method comprises the specific process: firstly, carrying out separation and purification of the crude extract of huperzia serrata by adopting an alkaline alumina column with the filler particle size of 100-200 mesh or 200-300 mesh, carrying out dry column packing with the diameter-height ratio of 1:8-1:30, carrying out dry sample loading with the sample loading ratio of 1:10-100, carrying out elution with chloroform-methanol (80:1-8:1), merging the flowing parts containing huperzine A, and packing a silica gel column to continue to separate. Silica gel column process parameters comprise: with the filler particle size of 100-200 mesh or 200-300 mesh, carrying out wet column packing with the diameter-height ratio of 1:8-1:30, carrying out wet sample loading with the sample loading ratio of 1:10-100, and carrying out elution with the chloroform-methanol (80:1-8:1). The obtained product is subjected to acetone recrystallization to obtain the finished product. The process route is economical and practical, and is convenient to industrialize. |
| priorityDate | 2012-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.