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filingDate 2014-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b6e9e7b34122536dfef3fa71238e27
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publicationDate 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103800347-A
titleOfInvention Combination drug for inhibiting proliferation of lung cancer cell, medicinal preparation and detection method
abstract The invention provides a combination drug for inhibiting proliferation of a lung cancer cell, a medicinal preparation and a detection method. The method comprises the following steps: selecting the lung cancer cell in an exponential growth phase, and digesting the lung cancer cell by adopting pancreatic enzymes; processing the lung cancer cell by adopting the combination drug composed of component 1 and component 2; simultaneously processing the lung cancer cell by adopting different concentrations of component 1, so as to form a plurality of group of contrast samples; adding a dye solution after treatment, and placing into an incubator to incubate and obtain bluish violet crystal formazan; removing supernate; dissolving the bluish violet crystal formazan by adopting dimethyl sulfoxide; detecting the survival rate of the cancer cell at the wavelength of 490nm by using a microplate reader; calculating the proliferation rate and the cloning number according to the survival rate, and respectively comparing the proliferation rates and the cloning numbers of different samples.
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