http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103792218-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2014-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103792218-B |
| titleOfInvention | Adopt double-core method to detect the genotoxic method of cigarette mainstream smoke total particulate matter |
| abstract | A kind of double-core method that adopts detects the genotoxic method of cigarette mainstream smoke total particulate matter, it is characterized in that: select human bronchial epithelial cell line BEAS-2B cell as appraisement system, cell is inoculated in culture dish, add cigarette smoke TPM sample to expose, the cell exposing is fixed through cultivation and methanol solution, adopt DAPI solution-dyed the counting of taking pictures under fluorescence microscope, in 1000 dikaryocytes of each sample counting, contain the cell quantity of micronucleus, compared with control sample, the quantity increasing by analyzing micronucleus, and then judge the size of smoke genetic toxicity. the target organ derived cell that the present invention adopts flue gas to act on human body carries out cigarette smoke genetic toxicity evaluation, and specific aim is stronger, in experimentation, without adding metabolism activation system (rats'liver S9 mixed liquor), fluorescent staining is directly carried out without being transferred to slide in Tissue Culture Dish, has the features such as easy and simple to handle, highly sensitive, reliable results. |
| priorityDate | 2014-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.