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filingDate 2014-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103789206-B
titleOfInvention Osteoblast electrical stimulation system based on microfluidic technology and operation method thereof
abstract The invention provides an operation method of an osteoblast electrical stimulation system based on a microfluidic technology. The osteoblast electrical stimulation system comprises a microfluidic chip, an inverted microscope, two elastic sealing plugs and an electrical stimulation signal source, wherein the microfluidic chip is prepared from a transparent material and is provided with a micro-channel which is used for bearing a cell culture medium and providing space environments for the culture of osteoblast, and an inlet and an outlet are respectively formed in the two ends of the micro-channel; a camera lens of the inverted microscope is aligned at the middle part of the micro-channel from the bottom of the microfluidic chip; the two elastic sealing plugs are used for respectively plugging the inlet and the outlet of the micro-channel; the electrical stimulation signal source is used for providing electrical stimulation signals, and the two electrodes of the electrical stimulation signal source respectively penetrate through the two elastic sealing plugs and then extend below the liquid level of the cell culture medium in the micro-channel. The osteoblast electrical stimulation system provided by the invention can be used for realizing the analysis of single osteoblast and assessing the influencing effect of different potential differences on the single osteoblast.
priorityDate 2014-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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