http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103749310-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12bfaed80c5e8f315ce8db8e3b5c84cd |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2014-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dd2ba922965561a08b15e78c74b4dee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d32eb4bbdd2ea31122000ee8b7397d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_652d3640b9289929b7350edabc490936 |
| publicationDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103749310-B |
| titleOfInvention | Method for promoting pinus massoniana tissue-cultured subcultured bud to root |
| abstract | The invention discloses a method for promoting a pinus massoniana tissue-cultured subcultured bud to root. The method comprises the following steps: selecting the robust and half lignified pinus massoniana tissue-cultured subcultured bud with a seedling height of 2-3 cm; by adopting different culture media in a two-step rooting method, inoculating the subcultured bud in a 1/2 modified MS culture medium IV that contains indoleacetic acids, indolebutyric acids and sucrose; conducting induction treatment on an adventitious root primordium 21-28 d; transplanting the subcultured bud to a 1/2 modified MS culture medium V that contains activated carbon or ascorbic acids, and sucrose, without auxins, in order to promote the adventitious root to express and extend; transplanting a rooted regeneration plant to a mixed matrix that contains yellow soil or peat soil, vermiculite and perlite for cultivating. According to the invention, the method can tremendously promote and improve the rooting condition of the subcultured bud, the rooting rate of the pinus massoniana subcultured bud is above 93.1%, and the highest rooting rate is as high as 97.6%; the root system quality is higher; the survival rate of transplanting is above 85.1%, and the highest survival rate of transplanting is as high as 93.0%; the method has good economic benefits and social benefits. |
| priorityDate | 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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