http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103740851-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2014-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103740851-B |
titleOfInvention | Based on hsa-miR-191-5p detection kit and the detection method thereof of AllGlo fluorescence probe quantitative PCR |
abstract | Based on hsa-miR-191-5p detection kit and the detection method thereof of AllGlo fluorescence probe quantitative PCR, relate to MicroRNA.Test kit is provided with box body, dividing plate, exogenous reference bottle, stem ring reverse transcription reagents bottle, real-time fluorescence quantitative PCR reagent bottle.Extract miRNA in sample, if serum/plasma or other body fluid samples, then does is the concentration that adding test kit after the abundant cracking of sample provides 5n? mol's is exogenous with reference to cel-miR-395 μ L, and whirlpool shakes; If cell or tissue sample, then exogenous with reference to cel-miR-39 without the need to adding; The stem ring reverse transcription reagents reverse transcription miRNA adopting test kit to provide is cDNA; CDNA is carried out real time PCR amplification by the real-time fluorescence quantitative PCR reagent adopting test kit to provide; Every data that composite analyser provides, set rational threshold value and baseline, carry out interpretation of result. |
priorityDate | 2014-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.