http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103740788-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18 |
| filingDate | 2014-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5bdf94d7e2841cc2837861175b11a82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb241fe2ce7f9fe9022bf14ecae663fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df2b1934d13c8e2e9ee0b5fa532d24b5 |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103740788-B |
| titleOfInvention | Method for preparing Globotriose and analogues of Globotriose through one-pot enzyme method |
| abstract | The invention discloses a method for preparing Globotriose and analogues of the Globotriose through a one-pot enzyme method. The method comprises the following steps: by using galactose or (2-deoxy galactose, 4-deoxy galactose and 6-deoxy galactose), lactose, UTP (uridine triphosphate), ATP (adenosine triphosphate), dithiothreitol, MgCl2, yeast inorganic pyrophosphatase and a Tris-HCl buffer solution as a reaction system, respectively adding galactokinase SpGalK, UTP-glucose-1-uridine phosphate invertase SpGalU and alpha-(1->4) galactosyl transferase LgtC for reaction; performing centrifugation and deproteinization, collecting a supernatant containing the Globotriose, and purifying through an activated carbon chromatographic column and a molecular sieve column, and performing freezing and drying to obtain the powdery Globotriose and the analogues thereof. According to the method, a main substrate is the galactose which is cheap and wide in source; the adopted enzymes SpGalK, SpGalU and LgtC are wide in substrate adaptability, and possibility is provided for mass quick production of the Globotriose and the analogues of the Globotriose. |
| priorityDate | 2014-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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