http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103739699-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-57581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2014-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103739699-B |
| titleOfInvention | Restructuring spotted deer antler extrasin beta 4and its production and use |
| abstract | The present invention relates to a kind of restructuring spotted deer antler extrasin betan 4 and its production and use, belong to gene engineering technology field.Described method comprises pilose antler extrasin betan 4 the clone of gene, prokaryotic expression carrier pET-28a-T βn 4 structure, at Host Strains E.coli? the steps such as the abduction delivering in BL21, the separation and purification of recombinant protein.The present invention utilizes genetic engineering technique to attempt first spotted deer antler extrasin betan 4 prokaryotic expression, successfully construct its prokaryotic expression carrier, and induce recombinant protein high expression, the recombinant protein content after purifying can reach 0.916mg/mL.Find through external activity research, restructuring spotted deer antler extrasin betan 4 obvious proliferation is had to scleroblast M3T3 and chondrocyte C5.18. |
| priorityDate | 2014-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.