http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103725771-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103725771-B |
| titleOfInvention | Quick and precisely detect the product vomitoxin of living in food simultaneously and do not produce vomitoxin bacillus cereus test kit and detection method thereof |
| abstract | The invention belongs to microorganism detection field, disclose a kind of method quick and precisely detecting product vomitoxin alive in food and do not produce vomitoxin bacillus cereus.Auele Specific Primer is designed for the cesB of bacillus cereus coding vomitoxin and the 16S rRNA of bacillus cereus, nitrine bromination third ingot (PMA) is adopted to eliminate the interference of the dead bacterium of bacillus cereus in food, boiling water bath method extracts viable bacteria DNA in food, carries out multiplex PCR detection.Add noncompetitive amplification interior label (IAC) and indicate the false negative result that may exist in PCR system.The false negative that present method is avoided molecular biology method to detect often having and false positive results interference, make detected result more accurate, present method is compared with classical culture protocols simultaneously, and detection time is shorter. |
| priorityDate | 2013-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.