http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103725748-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fac22dec1d0190ccd39375efa5280bed |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
filingDate | 2012-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b983701f12c93c29bd7b465c7ea4eb |
publicationDate | 2014-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103725748-A |
titleOfInvention | Method for measuring 1,5-anhydroglucitol by dehydrogenase |
abstract | The invention relates to a method for measuring 1,5-anhydroglucitol by dehydrogenase, which can be widely applied in the field of medical and biochemical technologies. The method comprises the following steps of: converting 1,5-anhydroglucitol (1,5-AG) to be measured and a main interferent, i.e., glucose (Glu), into 1,5-anhydroglucitol-6-phosphate (1,5-AG-6-P) and glucose-6-phosphate (Glu-6-P) by ATP dependent hexohinase (ATP-HK), respectively,and establishing an ATP regeneration system by piruvate kinase (PK) and phosphoenolpyruvate (PEP) in the system so as to ensure that 1,5-AG and Glu is converted into 1,5-AG-6-P and Glu-6-P, respectively. The converted 1,5-A-G-P is specifically dehydrogenated and oxidized by 1,5-anhydroglucitol-6-phosphate dehydrogenase (1,5-AG-6-PDH), and 2-(4-iodophenyl)-5-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazole is reduced to a colored substance and then quantified by a full automatic biochemical analyzer by colorimetry. |
priorityDate | 2012-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 293.