http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103725733-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-62 |
filingDate | 2013-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103725733-B |
titleOfInvention | Fermentation method of spinosad |
abstract | The invention discloses a fermentation method of spinosad. The fermentation method comprises the following steps: (1) performing slant culture and seed culture on a gene engineering strain LU104 which can improve the yield of the spinosad to obtain a seed solution; (2) inoculating the seed solution into a fermentation culture medium, and fermenting; adding stearic acid when fermentation is performed for 0-12h, adding isobutanol when the fermentation is performed for 40-56h, and adding glycine, alanine and valine when the fermentation is performed for 90-105h, wherein the fermentation is performed for 192-216h in total. According to the method disclosed by the invention, the highest yield of the spinosad can achieve 531.7mg/L. In addition, according to the culture method, each added substance has a single component, the targeting property is relatively strong, and the method further has certain guide significance for researching regulation and control processes in fermentation of the spinosad. |
priorityDate | 2013-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.