http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103712982-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-576 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate | 2013-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103712982-B |
titleOfInvention | A kind of highly sensitive TMB nitrite ion and preparation method thereof |
abstract | Nitrite ion multilist using TMB as chromogenic substrate on market reveals sensitivity this shortcoming not high, the invention provides a kind of highly sensitive TMB nitrite ion and preparation method thereof for this shortcoming.It is characterized in that, the concentration of each component is: nitrite ion A: trishydroxymethylaminomethane (Tris) is 12.11g/L, potassium ferrocyanide is 1g/L, sodium pyrophosphate is 5g/L, hydrogen peroxide is 1mL/L; Nitrite ion B:NaHn 2 pOn 4 2Hn 2 o is 2.96g/L, Nan 2 hPOn 4 12Hn 2 o is 29g/L, TMB is 5g/L, disodium ethylene diamine tetraacetate is 1-2g/L, dimethyl sulfoxide (DMSO) is 10mL/L, dodecyldimethylammonium hydroxide inner salt is 0.1-1g/L.Gain effect of the present invention greatly improves the sensitivity that euzymelinked immunosorbent assay (ELISA) detects hepatitis C, has very important practicality. |
priorityDate | 2013-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.