http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103712934-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-38 |
filingDate | 2014-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103712934-B |
titleOfInvention | A kind of for monitor laccase conformation change exempt from mark ultraviolet absorption spectroscopy |
abstract | The invention discloses a kind of for monitor laccase conformation change exempt from mark ultraviolet absorption spectroscopy.First 3-nitrine-umbelliferone is joined in the urea liquid of nine kinds of variable concentrations together with laccase, after fully reacting, use ultraviolet spectrometer (UVS) 400 ~ 700 again? the absorption spectrum of the above-mentioned mixed solution containing 3-nitrine-umbelliferone, laccase and urea is gathered within the scope of nm, finally draw the ultra-violet absorption spectrum changing trend diagram of laccase under different urea concentration condition, just can monitor laccase conformation change according to this ultra-violet absorption spectrum changing trend diagram.The present invention only needs laccase, 3-nitrine-umbelliferone simply to mix in urea liquid in operation, without any need for labeling process just can reach the object of monitoring laccase conformation change, greatly reduce the complicacy of operation, decrease monitoring time.In addition, the present invention is used for achieving comparatively satisfied result to the monitoring of laccase conformation change, demonstrates efficient, single-minded, timely, simple advantage. |
priorityDate | 2014-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.