http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103704135-B

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f948f322568997c75f58013e5b3dc52
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c0965ac74fff770d086c3d8306aa93b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a860318e90e643cf7bdfa3a7d51db47
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publicationDate 2015-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103704135-B
titleOfInvention In-vitro rapid propagation method for plantains
abstract The invention provides an in-vitro rapid propagation method for plantains. The in-vitro rapid propagation method for the plantains comprises the following steps: (1) callus induction culture, namely cutting off leaf veins and leaf margins of sterilized and disinfected plantains and cutting into small blocks; inoculating the plantains to a callus induction culture medium; and carrying out regular culture to form calluses and adventitious buds; and (2) rooting culture, namely selecting the adventitious buds, with the bud lengths of not more than 2cm-3cm, obtained in the step (1); cutting off the adventitious buds from the calluses and continuing to carry out callus induction culture on the residual calluses and the adventitious buds; and inoculating the cut adventitious buds on a rooting culture medium; and regularly culturing until white roots grow to obtain aseptic plantain germchits. According to the method provided by the invention, the disinfection efficiency of explants is up to 80% and the aseptic seedling rooting rate is 100%; the aseptic plantain germchits can be rapidly propagated; a plurality of problems that the propagation capability of plantain plants is low, the propagation period is long, resources are lacked and a lot of pesticides are remained are solved; the in-vitro rapid propagation method for the plantains has very important meanings in the aspects of protecting the natural resources of the wild plantains, protecting a good ecological environment and the like.
priorityDate 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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