abstract |
The invention provides nucleic acid, this nucleic acid comprises from 5 ' side successively toward 3 ' side: the genomic 5 ' non-translational region of hepatitis C virus J6CF strain; Comprise the encoding hiv protease district of NS3 albumen coded sequence, NS4A albumen coded sequence, NS4B albumen coded sequence, NS5A albumen coded sequence and NS5B albumen coded sequence; And 3 ' non-translational region, and during with the aminoacid sequence of the precursor protein of J6CF strain for benchmark, the L-Ala that above-mentioned NS4A albumen coded sequence has the 1680th is replaced to the sudden change of L-glutamic acid. |