http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103698418-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b45e0e78fe049a56c38f6da934012462 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2013-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ddce58a247fe5be6309fc07bb36d0016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fefbafe78647f59728240e74cdbeb9c6 |
| publicationDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103698418-B |
| titleOfInvention | Quantitative detection method for transgene protein CP4-EPSPS in plant |
| abstract | The invention relates to a quantitative detection method for transgene protein CP4-EPSPS in a plant. The quantitative detection method comprises: extracting protein from a plant to obtain a crude protein extraction solution; and carrying out digestion on the crude protein extraction solution, adopting three 18O-labeled CP4-EPSPS specific peptide segments as internal standards, adopting UPLC/ESI-QQQ MS detection, and carrying out quantification on CP4-EPSPS in a peptide segment level, wherein the three specific peptide segment sequences are ITGLLEGEDVINTGK, SFMFGGLASGETR and LAGGEDVADLR. According to the present invention, the method has high sensitivity, and absolute CP4-EPSPS quantification can be achieved through combination of an isotope dilution method and an MRM strategy. |
| priorityDate | 2013-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.