http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103694319-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 |
| filingDate | 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103694319-B |
| titleOfInvention | A kind of purification process of Buserelin |
| abstract | The present invention relates to a kind of purification process of Buserelin.Specifically, methods described includes：Thick peptide is dissolved with the methanol aqueous solution of volume ratio 5% -30%；Thick peptide solution is subjected to gradient elution purifying, the solution after adjusting pH value 5.5-6.5 using the aqueous solution triethylamine of the sulfuric acid of volume ratio 0.1%-0.4% and the perchloric acid of volume ratio 0.1%-0.4% is A phase, and acetonitrile is B phases, gradient：B%：10% -40% elution；The sample after gradient elution is turned by salt and desalination using chromatography in octadecylsilane chemically bonded silica chromatographic column, with 0.1 0.6%（w/v）Ammonium acetate aqueous solution is A phases, and acetonitrile is B phases, gradient：B% be 3% -10% gradient or it is isocratic rinse 15 30 minutes after, then with aqueous acetic acid acetonitrile system elute. |
| priorityDate | 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.