http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103667418-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d296e8ab6e760c611c1ed9fe05bc5f9f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab0bc7108ce4849c2888f64212daceb2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba2ccd153e51bf1ceb633e6e005427a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87263d50f0a00babdbb72beef224b2eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c050617e1273a2cc90c0e892673354cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f21cd1ae11d49ee43906909561980e10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1bb56eb360d6b9ad4cd39fb3d04ef0d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_007b065317e65e5afccfac952162a0e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47dd2643f4b571cbcd17ebb82ec63c78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ecd5ddf66fc33a2f765598ad5980e09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c18fd5fc8ac20f662fd5e1dea73464ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81b9085ac4801a984bc3afaafb4b165b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ada4ba8fe703d6b0b39d0f54feb28a5a |
| publicationDate | 2015-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103667418-B |
| titleOfInvention | High-throughput screening method of beta-lactam antibiotic synthetase with high activity |
| abstract | The invention discloses a high-throughput screening method of beta-lactam antibiotic synthetase. The method comprises the steps as follows: a synthetic liquid is added in plate holes of an ELISA (enzyme linked immunosorbent assay) plate, then a to-be-tested enzyme sample is added, the mixture reacts in a microplate oscillation reactor at the temperature of 10-30 DEG C for 0.1-3 hours, in the reaction process, the ELISA plate is taken out every 1-30 minutes, a PDAB (paradimethylaminobenzaldehyde) color development solution is added for color development, and a light absorption value in the position of 415 nm is measured through ELISA; according to variation trend of the light absorption values and a minimum value of the light absorption values, enzyme with high activity and good synthesis capacity is screened out; the synthetic liquid is prepared by dissolving mother nucleuses and side chains of beta-lactam antibiotics into a KPB solution; and the to-be-tested enzyme sample is beta-lactam antibiotic synthetase. The high-throughput screening method of beta-lactam antibiotic synthetase can be used for screening enzymatic synthesis enzyme of lactam antibiotics such as amoxicillin, cefalexin, cefaclor, cephadroxil, ampicillin and the like, has the characteristics of simplicity in operation, low cost, accurate detection and the like, and is suitable for large-scale high-throughput screening of synthesis enzyme. |
| priorityDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 92.