http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103667343-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103667343-B |
| titleOfInvention | A kind of transgenosis STEVIA REBAUDIANA genetic transforming method of agrobacterium rhizogenes mediation |
| abstract | A kind of transgenosis STEVIA REBAUDIANA genetic transforming method of agrobacterium rhizogenes mediation, in the agrobacterium rhizogenes bacterium solution for the AN DNA plasmids of pRI 101 that STEVIA REBAUDIANA stem apex explant immersion after preculture is carried target gene by this method, through infect, co-culture and bacteria removing after, by Hairy root culture on the screening and culturing medium containing kanamycins, the Hairy root of anti-kanamycins is formed on induction explant, induce Hairy root formation callus, break up adventitious bud, adventitious bud continued growth forms test tube seedling, and the transfer-gen plant for carrying target gene is identified using PCR amplification method.The technical scheme provided using the present invention, can be imported STEVIA REBAUDIANA by target gene, compared with the genetic transformation of the chemical methodes such as polyethylene glycol, reduce the link from Protoplast isolation and cultureļ¼Compared with the genetic transformation of the physical methods such as particle gun, it is not necessary to expensive instrument and equipment, and operating technology is simple. |
| priorityDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.