http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103667228-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2013-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103667228-B |
| titleOfInvention | Fungal nitrilase mutant that a kind of catalysis activity and heat endurance improve and construction method thereof |
| abstract | The present invention provides the Fungal nitrilase mutant and construction method thereof that a kind of catalysis activity and heat endurance improve, this mutant be by gibberella ( Gibberella intermedia ) CA3 1 Fungal nitrilase gene sets out, and uses many wheel fixed point saturation mutations to build mutant library and screen acquisition.The present invention uses the method for half design and rational to obtain Fungal nitrilase mutant, it is characterized in that the near Fungal nitrilase activated centre the 128th Ile and the 161st Asn is transformed, it is thus achieved that mutant be respectively Ile128Leu, Asn161Gln and Ile128Leu Asn161Gln.Mutant catalysis activity and heat endurance that the present invention provides all are significantly improved, and improved Fungal nitrilase mutant can reduce production cost, improve production efficiency, have higher using value. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636044-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636044-A |
| priorityDate | 2013-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.