http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103667205-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d927148c7982a59521f5c2ed8ae37c0 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-03001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2013-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e26f3b89c7f8a98c4c76481723698e8d |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103667205-B |
| titleOfInvention | Biologically labeled bimolecular site-directed biotinylated recombinant alkaline phosphatase (AP) |
| abstract | The invention discloses biologically labeled bimolecular site-directed biotinylated recombinant alkaline phosphatase (AP). The AP is constructed by biologically linking a biotin molecule to an amino terminal and a carboxyl terminal of the AP respectively. Through generation, the recombinant biotin-labeled enzyme is the bimolecular site-directed biotin-labeled recombinant AP. The enzymatic activity of the recombinant AP is not affected by a labeling factor. A construction method of the recombinant AP is characterized by firstly constructing an intermediate vector with two BirA substrate peptides (BSPs) and a protein linked flexible linker, then cloning an AP gene between the two BSPs by utilizing the PCR (polymerase chain reaction) technology, and obtaining the recombinant AP through expression of a bacterial expression system. The recombinant AP can form a reticular magnification structure with streptavidin or avidin in enzyme immunoassay of a BAS (biotin-avidin system), and can effectively improve the sensitivity and specificity of enzyme immunoassay of the BAS, reduce the dosage of the labeled enzyme and lower the detection cost. |
| priorityDate | 2013-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.