Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_815c016d95c536f756c63133972943fb |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16034 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0618 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
filingDate |
2013-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0ea4247d8de38fd5fa0d134d46f2ba7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e754103e0e2ce59f07a8137035b87e4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83451f8db5bcfce7c3799c2be5ec2abb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90367370c1e2cf8a50f4541e8baa5f58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78992d10685c6942fb16cc19d536f0f8 |
publicationDate |
2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-103667176-B |
titleOfInvention |
Carassius auratus gibelio brain tissue cell line sensitive to cyprinid herpesvirus II, and establishing method and application thereof |
abstract |
The invention discloses a carassius auratus gibelio brain tissue cell line sensitive to cyprinid herpesvirus II (CyHV-2) and an establishing method and application thereof. The cell line is preserved in China Center for Typical Culture Collection with an accession number of CCTCC No. C2013179. The carassius auratus gibelio brain tissue cell line has a good growth state and is sensitive to CyHV-2 which can be hardly cultured by using a common fish cell line; when CyHV-2 is continuously passed to a sixth generation on GiCB cells, viral nucleic acid can still be detected, and a cytopathic effect is stable; when an ultrathin electron microscopic section is prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and replication of the virions can be observed in the GiCB cells. The establishing method for the carassius auratus gibelio brain tissue cell line has good repeatability and scientific and reasonable conditions, is applicable to in vitro culture of CyHV-2 and provides a technical platform for research on separation, detection, culture and intact biological characteristics of CyHV-2. |
priorityDate |
2013-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |