| abstract |
The invention relates to a preparation method and application of a novel cationic graft copolymer and a multi-composite non-viral gene carrier. A novel cationic graft copolymer polycaprolactone-g-L polyethyleneimine (PCL-g-LPEI) was synthesized by ring-opening copolymerization and amidation reaction. The composition of its multiplex non-viral gene carrier includes: (A) plasmid DNA The polycaprolactone-g-L polyethyleneimine cationic complex acts as the core of the multiple gene complex; (B) the tumor-targeting ligand hyaluronic acid (HA) wraps the cationic complex through positive and negative charge interactions as the multiple gene complex Composite shell. The multiple compound gene constructed by this new type of non-viral gene carrier has the advantages of low cytotoxicity, good blood compatibility, high transfection efficiency, tumor targeting effect, etc., and is expected to be applied in clinical gene therapy. |