http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103665159-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 |
| filingDate | 2013-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103665159-B |
| titleOfInvention | The method of efficiently purifying quantum dot and IgG class monoclonal antibody conjugate |
| abstract | The invention discloses the new method of a kind of applicable scale efficiently purifying water-soluble quantum dot and IgG class monoclonal antibody conjugate, belong to biological technical field. For current quantum dot antibody coupling matter purifying process complexity; the rate of recovery is low; the drawback that is difficult to accomplish scale production; the present invention is by adopting the residual carboxyl of Glucosamine sealing quantum dot and antibody coupling matter; reduce the surperficial zeta potential of coupled product; by adjusting pH to 4.5～5.0, further reduce the net charge content of coupled product in solution, realize common supercentrifugal process scale efficiently purifying quantum dot and antibody coupling matter. The present invention has simplified the experimental implementation flow process of quantum dot and antibody coupling matter, reduce the requirement to separation equipment, be applicable to purifying quantum dot IgG class monoclonal antibody conjugate in enormous quantities, yield is more than 85%, and the fluorescent characteristic of coupled product and biologically active have no significant change. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108918858-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108918858-A |
| priorityDate | 2013-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.