http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103665150-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-43 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2012-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103665150-B |
| titleOfInvention | Pig β 2adrenoceptor fusion rotein and encoding gene thereof and expression method |
| abstract | The invention provides a boar beta 2 adrenoreceptor fusion rotein and encoding gene thereof and utilize silkworm biological reactor to express the method for this fusion rotein.The method mainly contains: (1) by the gene clone of the encoding fusion protein A of synthetic in Bac-to-Bac baculovirus expression system delivery vehicle; (2) by the specificity swivel base in site, the gene integration of the described encoding fusion protein A on the transfer expression vector obtained by step (1) is entered baculovirus plasmid; (4) by described baculovirus plasmid infected silkworm BmN cell, and with the silkworm larva in the baculovirus percutaneous puncture-inoculation 1-5 age obtained or pupal cell; (5) the silkworm larva expressed fusion protein B of the Bombyx noriN cell infected or inoculation.Encoding gene of the present invention carries out codon optimized obtaining for silkworm biological reactor, makes protein expression efficiency high, also for it has normal protein structure and biologic activity provides guarantee. |
| priorityDate | 2012-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 232.