http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103656679-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate | 2012-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103656679-B |
| titleOfInvention | A kind of preparation method of nano-particle |
| abstract | The invention provides a kind of preparation method of nano-particle, comprise the following steps: 1) polycation macromolecule be dissolved in ultra-pure water or be mixed with said polycation solution without RNase enzyme water, nucleic acid drug is dissolved in ultra-pure water or is mixed with nucleic acid solution without RNase enzyme water; 2) join in described nucleic acid solution by described said polycation solution, evenly, incubated at room temperature, obtains polyplexes in piping and druming repeatedly; 3) block macromolecular be dissolved in ultra-pure water or be mixed with block macromolecular solution without in RNase enzyme water, described block macromolecular solution is slowly added to described step 2) in the polyplexes granule prepared, piping and druming evenly, leave standstill, make it fully wrap up, the nano-particle wrapped up by block macromolecular can be obtained.Compared with prior art, nano-particle prepared by the present invention can get rid of the obstacle that cationic particle circulates in vivo, improves body-internal-circulation efficiency, meanwhile, also can grafting targeting group, realize sick cell targeting in body, and effectively improve Targeting Effect. |
| priorityDate | 2012-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.