http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103651334-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_849c97255c1c26a2bb46198a32aab19d |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
filingDate | 2013-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_100319de36cb3a1a7b7e2d55264344f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69a3c6a62258664a9d4c8360484103a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c37abe575ff9ed91ebb138685c9f5c4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b07964abfb1b1a8598432bab8923203d |
publicationDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103651334-B |
titleOfInvention | Luciola praeusta Kiesenwetter embryo vitrification cryopreservation method |
abstract | The invention discloses a Luciola praeusta Kiesenwetter embryo vitrification cryopreservation and unfreezing recovery method. The method comprises pretreatment of a Luciola praeusta Kiesenwetter embryo, shelling, five-step balanced treatment by mixing the embryo with cryoprotectant, cryopreservation of mixture after balanced treatment in liquid nitrogen, rewarming and incubation. By adopting the method provided by the invention to preserve the Luciola praeusta Kiesenwetter embryo, the survival rate of the Luciola praeusta Kiesenwetter is high, the reproduction rate after freezing is high and the method is suitable for the cryopreservation of embryos of different sub-breeds of Luciola praeusta Kiesenwetter. |
priorityDate | 2013-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.