http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103642828-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103642828-B |
| titleOfInvention | A kind of gene knock-in recombinant vectors and preparation method thereof and mouse model preparation method |
| abstract | The invention discloses a kind of recombinant vectors for gene knock-in, wherein, described recombinant vectors comprises DNA fragmentation structure I L17A-IRES-luc; Wherein, IL17A is the encoding gene of IL-17 A, and IRES is internal ribosome entry site, and luc is the encoding gene of secretor type luciferase.The preparation method that present invention also offers this gene knock-in recombinant vectors and the mouse model prepared with this recombinant vectors, this mouse model can be used for conveniently by the expression detecting secretor type luciferase in blood or urine thus detect the expression of IL17A gene; Do not need the complex apparatus such as fluorescent microscope; Secretor type luciferase is luminous by the oxidizing reaction of Luciferase catalyses substrate coelenterazine, and do not need exciting light, so fluorescence background is far below GFP fluorescence, and detection sensitivity is higher, and background is cleaner, and experimental result is also more accurate. |
| priorityDate | 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.