| abstract |
The invention discloses a sFLISA (sandwich fluorescence-linked immunosorbent assay) quick detection method and a detection kit of ochratoxin A based on quantum dots. The method comprises the following steps: enveloping an elisa plate which is black and has a transparent bottom with 100 microliters of 2.5 microgram/mL anti-OTA (ochratoxin A) polyclonal antibody; washing by using 200 microliters of 1*PBST, then adding 100 microliters of detected antigen sample solution; washing by using 200 microliters of 1*PBST, then adding 100 microliters of anti-OTA monoclonal antibody marked by biotinl, then adding 100 microliters of streptavidin marked by quantum dots and diluted according to a ratio of 1:100; washing and drying by using 200 microliters of 1*PBST, then detecting a relative fluorescence unit (RFU) by using a fluorescence spectrophotometer with an excitation wavelength of 390nm and an emission wavelength of 605nm, substituting the RFU of the sample into a standard curve to acquire the OTA content of the sample. The sFLISA quick detection method of ochratoxin A based on quantum dots has the advantages of accuracy, rapidness, good fluorescence stability, sensitivity and high specificity. |