http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103613623-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-07 |
filingDate | 2013-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103613623-B |
titleOfInvention | The application of multiple-stage solvent extraction in the synthetic isoquercitrin of enzymatic |
abstract | The application of multiple-stage solvent extraction in the synthetic isoquercitrin of enzymatic, it is that in glycine-sodium hydrate buffer solution solution of 9.0, concentration is 1g/L that rutin is dissolved in to pH, adding volume is 10% ionic liquid [Bmim] [BF 4 ] and hesperidinase liquid formation reaction system, at 40 DEG C, constant-temperature shaking culture obtains rutin hydrolyzate, and the pH of ionic liquid cosolvent system rutin hydrolyzate is between 2.0~7.0; Rutin hydrolyzate mixes with extractant, after constant temperature water bath concussion, carry out solvent extraction, compared with organic phase and water between 0.2~2.0, time is 10~150min, adopt afterwards multi-stage counter current extraction formula to calculate theoretical progression, reaching while extracting completely, filter out optimum extraction condition according to the extractant of required minimum ratio and theoretical stage. The method is easy and simple to handle, and technique is easily gone, and reduces difficulty and the cost of biological respinse later stage purifying, improves product recovery rate and purity. |
priorityDate | 2013-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.