http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103609451-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2013-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103609451-B |
| titleOfInvention | The preparation method of a kind of matrimony vine aseptic seedling and callus thereof |
| abstract | The invention discloses the preparation method of a kind of matrimony vine aseptic seedling and callus induction thereof, belong to field of plant tissue culture technique, it comprises the following steps: fruit is dry, seed disinfection: with containing 70% ethanol, the sterile distilled water of 0.1%Triton X-100 (Triton X-100) carries out seed disinfection, seed germination: the refrigerator being first placed in 4 DEG C, leave standstill 2 days, then moving to temperature is 25 ± 2 DEG C, the illumination every day culturing room of 14 hours cultivates 20 ± 2 days, callus squamous subculture: seed is positioned on subculture medium, under 25 ± 2 DEG C of conditions, cultivate in the illumination every day culturing room of 16 hours, every two weeks replaced medium.The method obtains the evoked callus of matrimony vine while obtaining matrimony vine aseptic seedling, simplifies this step of in Lycium barbarum callus Induction Process, it being carried out to physical trauma, shortens the cycle obtaining Lycium barbarum callus. |
| priorityDate | 2013-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.