http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103604922-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fce8ffbed915e3b126f6fc6eafd590f9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-135 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2013-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_444658091cd630195c1f4e2006d3ae07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8749fd1b1b0c36b588480bb13b57b19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a22dcdf3f3fb375564da1d93683dd33e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cd2ec673e9f51e9a1c6afbaf719ef87 |
| publicationDate | 2014-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103604922-A |
| titleOfInvention | Respiratory syncytial virus detection kit and use method thereof |
| abstract | The invention discloses a respiratory syncytial virus kit and a detection method thereof, belonging to the technical field of enzyme immunoassay. According to the respiratory syncytial virus kit, the pharyngeal of a child is wiped by adopting a throat swab, nasopharyngeal secretions of the throat swab are dissolved by an ambroxol hydrochloride medicine, nasopharyngeal epithelial cells as sediments are added in 2.0ml of a phosphate buffer solution containing 1.0 percent (V/V)Triton X-100 after being centrifuged at a high speed, stand for 30min, and are violently oscillated so that respiratory syncytial viruses fall off from the nasopharyngeal epithelial cells, a dissolved solution is taken to be added on a respiratory syncytial virus detection ELISA (Enzyme Linked Immunosorbent Assay) plate, the respiratory syncytial viruses are detected by adopting an ELISA method, warm bath and washing are carried out, a biotinylated respiratory syncytial virus antibody is added, streptavidin is coupled with a streptavidinbiotin peroxidase complex, and a substrate liquid is added, thus a result is read. The detection method is simple to operate, is rapid, sensitive and accurate, and is low in cost, is suitable for detecting the infection of nasopharyngeal epithelial cells by the respiratory syncytial viruses, and can be used as an important inspection index of infecting the respiratory syncytial viruses by the child. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104029900-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018000901-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106645678-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105759029-A |
| priorityDate | 2013-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.