http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103593659-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06K9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06K9-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06N3-02 |
filingDate | 2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103593659-B |
titleOfInvention | A kind of Sanger for diploid PCR primer is the recognition methods of individual internal SNP in checking order |
abstract | The invention discloses the recognition methods of an internal SNP during a kind of Sanger for diploid PCR primer checks order, be first separately separated out that the gland comprised in chromatogram looks sidelong at purine A, bird looks sidelong at purine G, cytosine C and the fluorescence data of tetra-kinds of bases of thymus pyrimidine T;Use wavelet analysis method that the fluorescence data separated is filtered denoising respectively;Analyze the wave character of four kinds of base fluorescence datas again, detect first peak and second peak of waveform, select crest distance, highly ratio and waviness ratio these three wave character, the key element differentiated as SNP site;Choice structure is the grader that detects as SNP site of BP neutral net of 3 10 1, and uses Levenberg Marquardt algorithm to be trained BP neutral net;Use piecewise linear transform that output is mapped as the SNP evaluation score of 0~100, be 1~5 grade according to evaluation score by the class declaration of SNP site, and judge the SNP confidence level in this site accordingly.The present invention can effectively detect the individual internal SNP site of order-checking file. |
priorityDate | 2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.