http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103592279-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103592279-B |
| titleOfInvention | A kind of assay method of bacterium born of the same parents glutathion inside content |
| abstract | The present invention discloses a kind of assay method of bacterium born of the same parents glutathion inside content, comprises the following steps: the typical curve drawing variable concentrations glutathione and fluorescence intensity; The method of plate culture count is adopted to carry out colony counting; Bacterium liquid experimental group to be measured and background fluorescence control group are set; After experimental group and control group process, fluorescence intensity detection method is adopted to detect; According to typical curve, calculate sample glutathione content, calculate sample bacterium quantity according to plate count; The ratio of glutathione content and corresponding bacterial number is the amount of a certain number of bacterium born of the same parents glutathion inside.The present invention adopts mBBr reagent, and it is by Gram-positive cell wall and enter cell and be combined with glutathione, generates stable fluorescent derivative, afterwards by acetonitrile cracking bacterium, solves the problem of gram positive bacteria broken wall difficulty in measuring method. |
| priorityDate | 2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 140.