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publicationDate 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103589728-B
titleOfInvention DNA (deoxyribonucleic acid) molecule for expressing hairpin type RNA (ribonucleic acid) for suppressing sitobion avenae carboxylesterase and application of DNA molecule
abstract The invention discloses a DNA (deoxyribonucleic acid) molecule for expressing a hairpin type RNA (ribonucleic acid) for suppressing sitobion avenae carboxylesterase and application of the DNA molecule. The structure of the DNA molecule disclosed by the invention is SEQ (forward)-X-SEQ (reverse); the sequence of the SEQ (forward) is from a locus 1 to a locus 345 of a sequence 1; the sequence of the SEQ (reverse) is reversely complementary to that of the SEQ (forward); the X is an interval sequence between the SEQ (forward) and the SEQ (reverse); according to the sequence, the X is not complementary to the SEQ (forward) and the SEQ (reverse). An experiment shows that the DNA molecule is converted into an immature embryo callus of wheat to obtain a transgenic line; the transgenic line is subjected to an aphid feeding test; after sitobion avenae is fed on transgenic leaves, the carboxylesterase gene expression quantity and the enzymatic activity are obviously alleviated, and the rate of reproduction is reduced; furthermore, the tolerance of the sitobion avenae to a phoxim solvent is reduced by 20-30 percent. Therefore, the DNA molecule has a great significance for prevention and treatment of insects of agricultural production.
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