http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103589704-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60d3043f1a2452f164ef76f35cb81e4c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 |
| filingDate | 2013-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2aea3e4c4b979abd28e7cfb4156da53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a06d1e5c864b0910f7d80199f69b56a8 |
| publicationDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103589704-B |
| titleOfInvention | Preparation method of Enteromorpha proteinase |
| abstract | The invention discloses a preparation method of Enteromorpha proteinase, which comprises the following steps: selecting raw materials, cleaning under high pressure, carrying out water leakage shearing, adding a proteinase inhibitor, juicing, adding an extract and a buffer solution, putting in a masher to prepare a crude extract, centrifuging the solid ammonium sulfate, dialyzing the acetic acid solution for desalting, adding a Roche proteinase inhibitor, cooling in an ice bath, adding chloroprene rubber cr322, stirring and centrifuging, purifying with sodium bicarbonate, crystallizing with a n-acetyl-l-cysteine solution, carrying out refrigerated centrifugation, collecting the crystal, and preserving the finished product. The method effectively ensures the activity and purity of the Enteromorpha proteinase. The method can be widely used in the fields of washing industry, food industry, textile industry, leather industry, paper pulp industry, feeds, petroligenic natural gas drilling, clinical medicine, agriculture, forestry, stock farming and the like, and has favorable effects of energy consumption reduction, chemical reagent reduction, high speed and environmental protection in application. |
| priorityDate | 2012-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.