http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103572378-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103572378-B |
titleOfInvention | Based on Ion Proton tMthe construction process of the small segment DNA library of order-checking platform and application thereof |
abstract | The invention belongs to gene sequencing field, disclose a kind of based on Ion? Protonn tM the construction process of the small segment DNA library of order-checking platform and relevant agent combination solution.Construction process of the present invention comprises the steps such as sample DNA extraction, end reparation, fragment screening, joint connection, pcr amplification, library detection and high-flux sequence.Connect at end reparation, joint and adopt reagent I, reagent II and reagent III respectively in pcr amplification step.Construction process step of the present invention is simplified, simple to operate, reagent type is few, cost is low, reduce the loss in library and waste, improve efficiency.In addition, construction process of the present invention can be used for based on Ion? Protonn tM the maternal blood foetal chromosome aneuploidy of order-checking platform without wound antenatal diagnosis, the high and safety economy of diagnostic result accuracy rate, can help the natality effectively controlling chromosome aneuploid fetus. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106867996-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106867996-B |
priorityDate | 2013-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.