http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103571950-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d76debd2ccd6ede2d8ade3908b76b1c |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1be3feedeb52b8bbc6f8cec7adb48d71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_439fbced53d16d156a4b04867e86ebdb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_447c376fdef76e3e66ccddab789a55be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2c76c0f27340ca2ea3d361882afb3c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_764579fe0a2f265714e363193998ee4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcf938bf2e4df9d56e31482073107b91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6bb668d6772eda6162267fa6880ddb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00498d2589c607bce54c4d67fdaa4584 |
publicationDate | 2015-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103571950-B |
titleOfInvention | Rapid detection kit for aeromonas schubertii and detection method |
abstract | The invention discloses a rapid detection kit for aeromonas schubertii. The rapid detection kit comprises a DNA (Deoxyribonucleic Acid) extraction reagent (1) and a PCR (Polymerase Chain Reaction) reagent (2), wherein the DNA extraction reagent comprises a TE (Tris-EDTA) buffer solution with 5-15mM of Tris and 0.5-1.5mM of EDTA (Ethylene Diamine Tetraacetic Acid), proteinase K with the concentration of 15-20mg/mL, 100% chloroform, 100% isopropanol, ethanol with the concentration of 70% and double distilled water; each PCR tube contains 2.5muL of 10*PCR buffer solution, 0.5muL of dNTPs with the concentration of 10muM, 0.3muL of Taq DNA polymerase with the concentration of 5U/muL, 0.5muL of specific oligonucleotide primers A with the concentration of 10muM, 0.5muL of specific oligonucleotide primers B with the concentration of 10muM, 19.2muL of sterilized deionized water, a primer A (5'-CAGCGAGGAGGAAAGGTTGGTGGT-3') and a primer B (5'-AAGCCACGCCTCAAGGGCACAA-3'). The rapid detection kit is used for detecting aeromonas schubertii. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108148815-A |
priorityDate | 2013-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.