http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103571925-B

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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156
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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
filingDate 2012-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac9a77842e87d3a98a87e5cc62a2ee50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ae2f5a4e75fcb02b7067df71b4c9c93
publicationDate 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103571925-B
titleOfInvention Specific detection primers and detection liquid phase chip for BIM gene mutation
abstract The present invention discloses a detection liquid phase chip and specific primers for BIM gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises deletion mutation-targeted SEQ ID NO.13, deletion mutation-targeted SEQ ID NO.14, A145G site-targeted SEQID NO.15, A145G site-targeted SEQ ID NO.16, C59T site-targeted SEQ ID NO.17, C59T site-targeted SEQ ID NO.18, T86C site-targeted SEQ ID NO.19, T86C site-targeted SEQ ID NO.20, G149C site-targeted SEQ ID NO.21, G149C site-targeted SEQ ID NO.22, and/or C104G site-targeted SEQ ID NO.23 and C104G site-targeted SEQ ID NO.24; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.
priorityDate 2012-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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