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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-03
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filingDate 2013-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b68a728f4a8148c6ff916d21c422977
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c78c942ce8a793f2bbe270049340995
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publicationDate 2014-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103571820-A
titleOfInvention Method for producing isoprene by adopting cell fusion technology and fusant constructed by same
abstract The invention discloses a method for producing isoprene by adopting a cell fusion technology and a fusant constructed by the same. The method comprises the following steps: screening by utilizing a protoplast fusion technology, taking escherichia coli and ralstonia eutropha as parents and taking lipid as a unique carbon source and a resistance maker plasmid to obtain the reconstituted cell of high-efficiency transformed lipid; and fermenting by utilizing the fusant to produce the isoprene. The method disclosed by the invention can increase the velocity of lipid metabolism, enhance the yield of the isoprene and reduce the fermentation cost.
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