http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103571784-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fefd66fdbc3870bbaa8e5a4f87bdb0a |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2013-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7674e3482b60edfd0c8ba7d2634cbcc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c650174516a2146b48b842e3182fe9a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2e06f7e2ac7fd4847dda34729ce21d9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c68bdcf9aeb69312e2da2c0d52a6efe6 |
| publicationDate | 2015-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103571784-B |
| titleOfInvention | Engineering bacteria of special lipase for expressing biodiesel and fermenting process of engineering bacteria |
| abstract | The invention provides engineering bacteria of special lipase for expressing biodiesel and fermenting process of engineering bacteria. The fermenting process comprises the following steps: by using onion Burkholderia GJ (B.cenocepacia) as original bacteria, screening constitutive strong promoter Pcon from the original bacteria through a fluorescent promoter probe carrier, cloning T7RNA polymerase gene not containing promoter in the promoter Pcon, feeding the promoter Pcon and the T7RNA polymerase gene into the original through electro-transformation to obtain host bacterial, cloning the lipase gene in a general carrier pBBR22b to obtain lipase expression plasmid, feeding the lipase expression plasmid into the host bacteria through the electro-transformation to obtain lipase engineering bacteria Burkholderiacenocepacia MTPF), CCTCC NO: M2O13229. The expression quantity of the lipase is greatly improved, and the produced lipase has good catalytic activity, low carbon alcohol tolerance and high temperature resistance. |
| priorityDate | 2013-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 146.