http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103555596-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7225909c974ca3ec710b40c423f25fbb |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
filingDate | 2013-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1553901e41e0215f2d064e3849254488 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3779d619e5ccca7a9563f0d321abc47f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06c9c6ccb350a441a9049f66ec958adc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49d849ef2ffd5866112a76a60a0a74ce |
publicationDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103555596-B |
titleOfInvention | Aspergillus niger strain for expression of lipase |
abstract | The invention aims to provide an aspergillus niger mutant strain (Aspergillus Niger WLlip1). The aspergillus niger mutant strain with a preservation number of CCTCC NO: M2013478 is preserved in China Center for Type Culture Collection of Wuhan University in China on October 18th, 2013. The aspergillus niger mutant strain provided by the invention can carry out efficient recombinant expression on lipase, the protein expression quantity of the mutant strain is increased by about 40% in comparison with that of pre-mutant strains, the lipase enzyme activity of fermentation liquor of the mutant strain is up to 5482 U/mL and increased by 78% in comparison with that of pre-mutant strains, the enzymatic property of lipase subjected to recombinant expression after mutation is not changed due to mutation, the optimal operative pH value is 9.5, and the optimal operative temperature is 35 DEG C. Lipase subjected to recombinant expression by the WLlip1 mutant strain has a strong decontamination effect on corticosteroid dirt, therefore, the aspergillus niger mutant strain can be widely applied to the field of detergents. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106467899-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106467899-A |
priorityDate | 2013-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.